Thp 1 cell line transfection

2020-01-27 11:32

I have tried to transfect THP1 cells (human monocytic cell line) with a plasmid containing the GFP reporter gene but I could not get a high transfection efficiency.Sep 02, 2014 This approach allows for the transfection of cell lines such as human THP1 monocytes and macrophages and has been successfully applied in the past 610. In contrast to most chemical transfection approaches, our modified Nucleofection procedure using premature macrophages yields high transfection efficiencies in combination with unimpaired cell thp 1 cell line transfection

How high is the transfection efficiency? I have tried to transfect THP1 cells (human monocytic cell line) with a plasmid containing the GFP reporter gene but I could not get a high transfection

vitro gene transfection inanhum suspensioncell lines especiallythe monocytic cell line THP1, which is notorious for its low efficiency of transfection. THP1 cells transfected with the lipidshowed F considerably lower transfection efficiencies than those transfected with METAFECTENE. THP1 cells are recalcitrant to cationic and lipidbased methods of transfection, meaning I was unable to use a Lipofection protocol. Protocol for recombinant Cas9 NLS Nuclease protein to edit the genome of a THP1 cell line: 1. Add 1 ug of Cas9 nuclease preloaded with 250 ug of validated (in HEK293M) sgRNA to form the ribonucleoprotein thp 1 cell line transfection Transfection is a useful tool to study the function of gene products, but transfection of THP1 monocytes and predifferentiated THP1 macrophages with subsequent differentiation into mature THP1 macrophages using phorbol esters is usually accompanied by a progressive loss of cell viability.

The Cell Line NucleofectorTM Kit V is for transfection of cell lines, e. g. HepG2, HL60, Jurkat, K562, MCF7, SHSY5Y, or THP1. thp 1 cell line transfection Note: Invitrogen Lipofectamine Transfection Reagent Protocols have been optimized for efficiency, viability, and reproducibility across a broad range of cell types. This is often the best place to start, especially in a new cell line. If you find this doesnt work for your specific cell type, then Page 1 of 3GeneXPlus Transfection of Plasmid DNA into THP1Cells THP1(ATCC No. TIB202) is a human acute monocytic leukemia cell line that was derived from peripheral blood. ATCC achieved transfection efficiencies of approximately 38 with 90 cell viability using the protocol described below. THP1 is a human monocytic cell line derived from an acute monocytic leukemia patient. It is used to test leukemia cell lines in immunocytochemical analysis of proteinprotein interactions, and Characteristics. Although THP1 cells are of the same lineage, mutations can cause differences as the progeny proliferates. The base medium for this cell line is ATCCformulated RPMI1640 Medium, Catalog No. . To make the complete growth medium, add the following components to the base medium: 2mercaptoethanol to a final concentration of 0. 05 mM; fetal bovine serum to a final concentration of 10.

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