Bleomycin induced lung fibrosis protocol

2020-01-22 08:26

Bleomycin. Bleomycin is a chemotherapeutic antibiotic, produced by the bacterium Streptomyces verticillus (Adamson, 1976; Umezawa, 1967). Its use in animal models of pulmonary fibrosis is based on the fact that fibrosis is one of the major adverse drug effects of bleomycin in human cancer therapy.Jun 13, 2017  Effect of felodipine on bleomycininduced pulmonary fibrosis, alteration of lung mechanics, and respiratory dysfunction. Mice were intratracheally administered with bleomycin bleomycin induced lung fibrosis protocol

Lung fibrosis was induced in mice by intratracheal administration of bleomycin (BLM) at 0. 25, 0. 5, 0. 75 and 1 mgkg. A detailed characterization of BLMinduced fibrosis was performed 14 days after BLM administration using lung function testing, microcomputed tomography and Ashcroft scoring analysis.

Alterations in pulmonary mRNA encoding procollagens, fibronectin and transforming growth factorbeta precede bleomycininduced pulmonary fibrosis in mice. J Pharmacol Exp Ther 1988; 246: 765. Khalil N, Whitman C, Zuo L, et al. Regulation of alveolar macrophage transforming growth factorbeta secretion by corticosteroids in bleomycininduced Mar 01, 2004 Tsuburai and colleagues have reported that adenovirus mediated transfection of HO1 can attenuate the intensity of bleomycin induced pulmonary fibrosis. 27 However, their study differs from ours in that unequivocal HO1 protein expression was induced before bleomycin treatment, with levels peaking approximately 1 week after the instillation of bleomycin induced lung fibrosis protocol Bleomycininduced Lung InjuryPulmonary Fibrosis (IPF) Bleomycin is a chemotherapeutic agent used to treat cancers such as Hodgkins lymphoma. One of the side effects is pulmonary toxicity, which can be life threatening in approximately 10 of patients.

In bleomycininduced lung fibrosis models, the fibrotic lesions occur 14 21 days after bleomycin administration. In the presented work, we used a new protocol to induce lung fibrosis in mice by double bleomycin intratracheal instillation. bleomycin induced lung fibrosis protocol mashed food to help prevent excess weight loss, and all lung function assessments and tissue collections were performed 21 days postdosing unless explicitly stated otherwise. (Male Sex Hormones Exacerbate Lung Function Impairment After BleomycinInduced Pulmonary Fibrosis, AJRCMB Articles in Bleomycininduced lung fibrosis is a widely used mouse model for human idiopathic pulmonary fibrosis. Ddr1 mice were largely protected from bleomycininduced lung injury, and similar to the situation with chronic renal disease, DDR1 mediated both inflammation and fibrosis in Mar 01, 2008 Pulmonary fibrosis is a component of many interstitial lung diseases, including idiopathic pulmonary fibrosis, a chronic, progressive disease for which there is currently no effective therapy. Bleomycin has been widely used in rodents to model pulmonary fibrosis for the study of mechanisms involved in fibrogenesis and for evaluation of Bleomycin is a chemotherapeutic agent commonly used to treat curable diseases such as germinative tumors and Hodgkins lymphoma. The major limitation of bleomycin therapy is pulmonary toxicity, which can be life threatening in up to 10 of patients receiving the drug. The mechanism of bleomycininduced pneumonitis (BIP) involves oxidative damage, relative deficiency of the deactivating

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